Fig. 7: Tolerance of MPI 8 in mice.
From: Smart thrombosis inhibitors without bleeding side effects via charge tunable ligand design
A–C MPI 8 were well tolerated in mice at high doses. A–C LDH activity, ALT activity, AST activity in mice, respectively, after injecting MPI 8 intravenously. Mice were sacrificed after 24 h (acute study). D Schematic representation of mouse chronic toxicity model. Female BALB/c mice were administered either saline or escalating doses of MPI 8, up to 500 mg/kg. Mice were monitored daily and body weights were measured. After 15 days, serum was collected from sacrificed mice and analyzed for LDH levels. Mice injected with MPI 8 showed no significant change in body weight compared to mice injected with saline and no increase in LDH levels. E Change in body weight over 15 days. F LDH activity. G Representative stained (H&E) images of organs collected from mice injected (i.v.) with 500 mg/kg MPI 8. No abnormalities were seen in the heart, lungs, liver and kidneys of mice administered with 500 mg/kg MPI 8 compared to the saline control. All results (A–C, E–F, G) represent mean values +/− SD of n = 4 mice per group. Figure 7D was created using BioRender.com.
