Fig. 4: ENL co-factors and MYST family KATs determine survival in leukemia cells.
From: MOZ/ENL complex is a recruiting factor of leukemic AF10 fusion proteins
a Requirement of DOT1L, ENL, MOZ, MORF, and HBO1 for myeloid progenitors immortalized by CALM-AF10 or MLL-ENL. sgRNA competition assays of immortalized myeloid progenitors were performed. The ratio of GFP-positive cells co-expressing sgRNA was measured using flow cytometry. sgRNA for Renilla luciferase (Ren), which does not affect proliferation, was used as a negative control. sgRNA for Rpa3, which impairs proliferation, was used as a positive control. b Requirement of DOT1L, ENL, MOZ/MORF, and HBO1 for human leukemia cell lines carrying CALM-AF10 (P31/FUJ) or MLL-ENL (HB1119). sgRNA competition assays were performed in P31/FUJ and HB1119 cells as in a. Source data are provided as a Source Data file.
