Fig. 7: Inhibition of MOZ/MORF KATs reduces ENL-mediated oncogene activation in CALM-AF10 leukemia cells.
From: MOZ/ENL complex is a recruiting factor of leukemic AF10 fusion proteins
a Genomic localization of ENL. ChIP-seq for ENL was performed on P31/FUJ and HEK293T cells. Data for RNA Polymerase II with its CTD phosphorylated at Ser 5 of the heptapeptide repeats (RNAP2-Ser5-P) are shown as a comparison. b Gene expression after MOZ/MORF KAT or DOT1L KMT inhibition. RT-qPCR was performed on the P31/FUJ cells treated with 10 μM WM1119 (W), 10 µM EPZ-5676 (E), or DMSO control (D) for 3 or 6 days (mean ± SD of technical replicates, n = 3). c Effects of MOZ/MORF KAT inhibitor on the localization of ENL, ING4, Cyclin T1, and RNAP2-Ser5-P at target chromatin. ChIP-qPCR was performed on P31/FUJ cells treated with WM1119 (W) (1 μM for 24 h) or DMSO control (D) (mean ± SD of technical replicates, n = 3) using specific antibodies for the indicated proteins. The positions of the qPCR probe are set at 0.5 kb upstream and 1.5 kb downstream of TSS as indicated on the top. Source data are provided as a Source Data file.
