Fig. 2: SARS-CoV-2 S WA1/2020, BA.1-, and BA.2-specific neutralizing antibody responses to Ad26.COV2.S, Ad26.COV2.S.529, or a combination of Ad26.COV2.S and Ad26.COV2.S.529 late booster in adult rhesus macaques. | Nature Communications

Fig. 2: SARS-CoV-2 S WA1/2020, BA.1-, and BA.2-specific neutralizing antibody responses to Ad26.COV2.S, Ad26.COV2.S.529, or a combination of Ad26.COV2.S and Ad26.COV2.S.529 late booster in adult rhesus macaques.

From: Booster with Ad26.COV2.S or Omicron-adapted vaccine enhanced immunity and efficacy against SARS-CoV-2 Omicron in macaques

Fig. 2

SARS-CoV-2 neutralizing antibody titers were measured over time in NHP serum samples (42 NHPs and 7 time points) with a luciferase-based psVNA specific for the S glycoprotein of SARS-CoV-2 of the a WA1/2020 strain (Wuhan/WIV04/2019, GISAID accession ID: EPI_ISL_402124 [https://gisaid.org/wiv04/]), b Omicron BA.1 strain (GISAID accession ID: EPI_ISL_7358094.2 [https://gisaid.org]), and c Omicron BA.2 strain (GISAID accession ID: EPI_ISL_6795834.2 [https://gisaid.org]). Neutralizing antibody responses were expressed as the reciprocal of the sample dilution where 50% neutralization was achieved (NT50). Neutralizing antibody levels in individual animals are depicted with gray points and paired measurements connected with gray lines. The GMT of neutralizing antibody responses per group is indicated with the red line. The horizontal dotted line indicates the LLOD. The red arrows indicate the time of challenge (week 6). For all panels, n = 7 animals per group except for the Sham-Ad26.COV2.S.529 group where n = 6 animals and for the Sham-Sham group where n = 8 animals. Comparisons between specific vaccine groups were made in a Tobit ANOVA with a post hoc z- or t-test. Source data are provided as a Source Data file. ANOVA, analysis of variance; GMT, geometric mean titer; LLOD, lower limit of detection; NHP, nonhuman primate; NT50, 50% neutralization titer; psVNA, pseudovirus neutralization assay; S, spike.

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