Fig. 5: Endogenously produced H2O2 impairs ATP produced in the mitochondria. | Nature Communications

Fig. 5: Endogenously produced H2O2 impairs ATP produced in the mitochondria.

From: IDH3γ functions as a redox switch regulating mitochondrial energy metabolism and contractility in the heart

Fig. 5

A ATP levels in HEK HyPer-DAO-NLS and wild type (wt) cells either non-treated (n.t.) or after treatment with 50 mM L-ala and D-ala ± 8 mM NAC or 500 µM H2O2 for 20 min, n = 8 independent samples. B Mitochondrial and glycolytic ATP production in HEK HyPer-DAO-NLS cells after treatment with 50 mM L-ala or D-ala for 20 min. n = 4 independent experiments. C Mitochondrial and glycolytic ATP production in cardiomyocytes isolated from HyPer-DAO mice after treatment with 2 mM D-ala or L-ala for 20 min. n = 7 independent mice per group. D Ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) and total glutathione levels E in HEK HyPer-DAO-NLS cells either n.t. and after treatment with 50 mM D-ala or L-ala for 20 min, n = 3 biologically independent samples. Data are presented as mean values ± SEM, one-way ANOVA (A, B, C, D and E). Source data are provided as a Source Data file.

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