Fig. 1: Lineage-tracing-based imaging shows altered fibroblast quantity and distribution in inflamed mouse skin.

a Fibroblast lineage-tracing strategy of Pdgfra^DreER-tdTomato mice. Flow cytometric analysis (b) and quantification (c) of tdTomato labeling efficiency in dermal PDGFRa⁺ fibroblasts of untreated (UT) and imiquimod (IMQ)-induced mouse skin (n = 3). d Representative whole-mount images of untreated Pdgfra^DreER-tdTomato mouse skin. Scale bar, 100 μm. e Schematic diagram of IMQ-induced psoriasis mouse model in self-control Pdgfra^DreER-tdTomato mice with different tamoxifen dosages. Luminescence imaging (f) and quantification (g) of tdTomato intensity in IMQ-induced self-control Pdgfra^DreER-tdTomato mice (n = 5). Representative hematoxylin and eosin (H&E, h) and immunofluorescent (i) images of UT or IMQ-induced Pdgfra^DreER-tdTomato mouse skin (n = 5). Scale bar, 50 μm. Data are representatives of two independent experiments. Data in (c) are presented as the mean ± SEM. The P values were calculated by two-tailed paired Student’s t-test. Source data are provided as a Source Data file.