Fig. 2: A tenascin-C (TNC)+ fibroblast subpopulation emerges at the dermal-epidermal junction (DEJ) of psoriatic skin.

UMAP plot (a, b) and cluster dendrogram (c) of Pdgfra-lineage cells sorted from back skin of UT and IMQ-induced Pdgfra^DreER-tdTomato mice. d Violin plots of cluster 9 signature genes expression. e qPCR analysis of Pdgfra, Coch, and Tnc in the dermis of UT or IMQ-induced mice (n = 4). f Immunoblot of Cochlin and TNC expression in the dermis of UT or IMQ-induced mice (n = 5). g Representative immunofluorescent images of Cochlin (upper panels) and TNC (lower panels) expression in UT or IMQ-induced mice (n = 5). Scale bar, 50 μm. h Top-ranked Gene Ontology (GO) pathways of Coch⁺Tnc⁺ fibroblast-enriched genes. i Representative spatial transcriptome images of TNC expression in normal (n = 2) or psoriatic human skin (n = 3). j Representative immunofluorescent images of TNC expression in non-lesional /marginal/lesional skin of human psoriatic skin (n = 5). Scale bar, 100 μm. Data are representatives of two independent experiments. Data in (e) are presented as the mean ± SEM. The P values were calculated by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.