Fig. 5: Fibroblast-specific ablation of Tnc restrains skin inflammation and excessive axonogenesis in mouse psoriasiform lesions.
From: Tenascin C+ papillary fibroblasts facilitate neuro-immune interaction in a mouse model of psoriasis
a Change in fold-back skin thickness of IMQ-induced Tncfl/fl (male: n = 5; female: n = 5) or Col1a2CreERTncfl/fl (male: n = 5; female: n = 4) mice. Representative H&E staining images (b) and quantification of skin acanthosis (c) of IMQ-induced Tncfl/fl (male: n = 5; female: n = 5) or Col1a2CreERTncfl/fl mice (male: n = 5; female: n = 4). Scale bar in (b), 50 μm. Representative immunofluorescent images (d) and quantification (e) of Ki67+ cells in the epidermis of IMQ-induced Tncfl/fl or Col1a2CreERTncfl/fl mice (n = 5). Scale bar in (d), 50 μm. Quantification of CD45+ immune cells (f) and CD3+ T cells (g) in the skin of IMQ-induced Tncfl/fl or Col1a2CreERTncfl/fl mice (n = 5). Representative immunofluorescent images (h) and quantification (i) of intra-epidermal nerve fiber (IENF) intensity in the skin of IMQ-induced Tncfl/fl or Col1a2CreERTncfl/fl mice (n = 5). Scale bar in (h), 50 μm. j Representative whole-mount images of nerve fibers in the skin of IMQ-induced Tncfl/fl or Col1a2CreERTncfl/fl mice (n = 3). Scale bar, 30 μm. Data are representatives of two independent experiments and presented as mean ± SEM. The P values were calculated by two-way ANOVA and Holm–Šídák test (a) or two-tailed unpaired Student’s t-test (c, e, f, g, i). Source data are provided as a Source Data file.
