Fig. 2: Tolerance of SULI to fusion at different positions of the target protein. | Nature Communications

Fig. 2: Tolerance of SULI to fusion at different positions of the target protein.

From: Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction

Fig. 2

The photoswitchable degrons SULI, AuLOV-cODC1, and psd3 were fused to the C- a, b or N-terminal (c, d) or the inner region (e, f) of the reporter protein. Yeast cells expressing these fusion proteins were cultured upon blue light illumination or under dark conditions for 15 h before the fluorescence was imaged by fluorescence microscopy (a, c, e) and quantified by a microplate reader (b, d, f). The fluorescence was normalized to that of the cells expressing reporters without fusion with the photoswitchable degrons under dark conditions. The scale bars in a, c, and e are 50 μm. The data in b, d, and f are presented as the mean ± SD from three biological replicates. Source data are provided as a Source Data file.

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