Fig. 3: Kinetics characteristics of SULI.

a Degradation kinetics of SULI-regulated protein stability. Yeast cells expressing different SULI fusion proteins cultured upon light illumination were then incubated with CHX and transferred to dark conditions or kept in light conditions. The fluorescence of the fusion proteins at the indicated time points was recorded by flow cytometry. The data were normalized to the fluorescence at time 0 min. b Oscillatory control of protein abundance by SULI. Yeast cells expressing mCherry-SULI were cultured alternatively in light and dark with an interval of 5 h for a total of 20 h. mCherry fluorescence at the indicated time points was measured using a microplate reader. The data were normalized to the data for the cells expressing mCherry alone at each indicated time point. The data are presented as the mean ± SD from three biological replicates. c Light irradiance-dependent regulation of protein stability by SULI or AuLOV-cODC1. The data were collected by flow cytometry and normalized to the data for the cells expressing mCherry alone under each indicated blue light condition. The data in a–c are presented as the mean ± SD from three biological replicates. d Spatial control of protein abundance by SULI. Yeast cells expressing mCherry-SULI were grown on solid medium and irradiated by blue light using a mask with a specific image (left panel). Imaging of mCherry fluorescence was performed (right panel). The orange circle indicates the glass bottom of the plate to which the cells were attached. Scale bar, 1 cm. Source data are provided as a Source Data file.