Fig. 5: Optical control of the cell cycle and growth.

a Schematic representation of the SULI-controlled stability of ^ΔNSic1. SULI was fused to the C-terminus of ^ΔNSic1, whose SCF^Cdc4-dependent degradation sequence (amino acids 1–105 of Sic1) was deleted. b Yeast cells expressing ^ΔNSic1-SULI were serially diluted (1:10; first spot, ~2.5 × 10⁴ cells) and grown in solid medium under light or dark conditions. BY4742 cells transformed with empty vectors were used as the controls. c The same yeast cells as in b were cultured under light or dark conditions for 10 h before imaging. The circular graphs (inner ring, dark conditions; outer ring, light conditions) show the mean distribution of cell cycle stages obtained from three biological replicates. At least 100 cells were counted for each replicate. Propidium iodide was used to stain the DNA of yeast cells (red). Scale bars, 5 μm. d Schematic representation of SULI-mediated degradation of endogenous ADE2 expressed from the genomic gene. A DNA fragment containing the SULI-encoding gene and a LEU2 expression cassette was integrated into the C-terminus of the genomic ADE2 gene. mCherry was used to replace SULI as the control. e Yeast cells expressing ADE2-SULI or ADE2-mCherry were serially diluted (1:5; first spot, ~2.5 × 10⁴ cells) and grown in the solid medium under light or dark conditions.