Fig. 6: Optical control of protein stability by SULI in zebrafish.

a Schematic representation of SULI-mediated degradation of the protein of interest (POI) in zebrafish embryos. mRNA encoding SULI fused with a protein of interest was transcribed in vitro using the SP6 promoter and injected into the zebrafish embryos. b Image of mCherry and EGFP fluorescence in zebrafish embryos injected with mRNAs encoding mCherry-SULI and EGFP or EGFP-Hsp104. mRNA encoding mCherry alone was used to replace mCherry-SULI as the control. All the zebrafish embryos were incubated in E3 medium supplemented with 5 μM FAD. Scale bar, 200 μm. c Quantitative analysis of mCherry fluorescence in zebrafish embryos. The data are presented as the mean ± SD. Statistical comparison was performed by two-tailed t test. n = 20, 19, 63, 56, 49, 44, 44, 52, 40, 42 zebrafish embryos from left to right, respectively. N.S., no significant difference. a.u., arbitrary units. d Optical control of the development of zebrafish embryos by SULI. Zebrafish embryos injected with mRNA encoding Pitx2-SULI and Hsp104 were incubated under light or dark conditions for 24 hours before images were taken. mRNA encoding SULI and Hsp104 was used as the control. The white arrows indicate the malformed embryos. Scale bar, 200 μm. e Enlarged figures to show the malformation or normal phenotype of the injected zebrafish expressing Pitx2-SULI kept under light or dark conditions, respectively. Scale bar, 500 μm. f Quantitative analysis of the development of zebrafish embryos under different conditions. The data are the results from >100 embryos for each group. Source data are provided as a Source Data file.