Fig. 2: ExTxA does not inhibit NaV1.7 inactivation heterologous expression systems.
From: Pain-causing stinging nettle toxins target TMEM233 to modulate NaV1.7 function
a Persistent current (I40 ms/Ipeak) induced by ExTxA (1 μM) in TE-671 cells, HEK293 cells stably expressing β1 and hNaV1.1, hNaV1.2, hNaV1.3, hNaV1.4, hNaV1.5, hNaV1.6, hNaV1.7 or CHO cells expressing hNaV1.8/β3, ND7/23 cells, F11 cells, SH-SY5Y neuroblastoma cells and Xenopus oocytes expressing hNaV1.7. b Representative control (black) and ExTxA-treated (1 μM, colored) normalized current traces of data shown in (a). Current was elicited by a depolarizing step to −20 mV (−10 mV for oocytes) from a holding potential of −90 mV. Scale bar: all 1 nA, 5 ms except oocytes (1 µA, 5 ms). c ExTxA (1 μM) effect on persistent current (I40 ms/Ipeak) in CHO cells expressing hNaV1.7 with or without the β1, β2, β3 or β4 subunit. d Representative control (black) and ExTxA-treated (1 μM, green) current traces of data shown in (c). Inset: Expression of GFP confirming successful transfection of the GFP-IRES β subunit plasmids. Scale bar, 10 µm. Data are shown as mean ± SEM; n.s., not significant; *,#p < 0.05. n values and statistical information are detailed in Supplementary Table 1. Source data are included as a Source Data file.
