Fig. 6: Effects of TMEM233 on NaV1.7 function.
From: Pain-causing stinging nettle toxins target TMEM233 to modulate NaV1.7 function
a Current-voltage relationship and (b) family of sample traces from hNaV1.7 (n = 6) co-expressed with TMEM233 (n = 11) in HEK293 cells. c Superimposed conductance–voltage (G–V, squares) and steady-state fast inactivation (circles) curves, of mock- (control, yellow) and TMEM233-transfected (teal) HEK293-NaV1.7 cells. d V50 of activation and (e) V50 of inactivation of mock- and TMEM233-transfected HEK293-NaV1.7 cells. f Voltage-dependence of slow inactivation and (g) V50 of slow inactivation in mock- and TMEM233-transfected HEK293-NaV1.7 cells. h Time-dependence and (i) time constant τ1 of recovery from fast inactivation in mock- and TMEM233-transfected HEK293-NaV1.7 cells. j Peak currents (Imax, nA) and (k) representative traces of currents elicited by slow ramp depolarizations (−100 mV to +20 mV at 1 mV/s) in control and TMEM233-transfected HEK293-NaV1.7 cells. l Peak currents of the last depolarization (30th pulse), normalized to the first pulse, from control and TMEM233-transfected HEK293-NaV1.7 cells at pulse frequencies of 1, 2, 5, 10, and 20 Hz. m Left: Time constant τ1 of recovery from fast inactivation in HEK293-NaV1.7 cells that were mock-transfected (control), transfected with full-length TMEM233 (TMEM233) or N-terminally truncated TMEM233 lacking residues 1–34 (del. 1–34). Right: Time constant τ1 of recovery from fast inactivation in HEK293-NaV1.7 cells with buffer control (0.1% BSA) or synthetic TMEM233 N-terminal peptide (100 μM) in the intracellular solution (ICS). Data are shown as mean ± SEM; n.s., not significant; *p < 0.05. n values and statistical information are detailed in Supplementary Table 1. Source data are included as a Source Data file.
