Fig. 5: The dimeric mechanism of Mtb-RNase J.

a Superimposition of the gel filtration profiles of Mtb-RNase J alone or with 10 mM EDTA or Mn²⁺ on a Superdex200 10/300 column. b Overview of the interacting interfaces of the two Mtb-RNase J chains. Chains A and B were colored cyan and orange, respectively. The detailed residues were shown in the sticks. c–e Enlarged view of the interacting interface, corresponding to (b). The detailed residues were shown in stick models. f Gel filtration profiles of Mtb-RNase J and its different truncations on a Superdex200 10/300 column. g Ribbon representation of Mtb-RNase J/ssRNA structure, with different numbers denoting the truncated sites. h The ribonuclease activities of wild type (WT) and different truncations of Mtb-RNase J.