Fig. 6: Negatively charged regions 46-64 and 440-450 of Mtb-RNase J were crucial for RNA binding. | Nature Communications

Fig. 6: Negatively charged regions 46-64 and 440-450 of Mtb-RNase J were crucial for RNA binding.

From: Structural insights into RNase J that plays an essential role in Mycobacterium tuberculosis RNA metabolism

Fig. 6

a The overall electrostatic distribution of the Mtb-RNase J/ssRNA complex. The yellow box represented the substrate-entrance pocket. Red: negative potential; blue: positive potential. b The surface electrostatic potential of region 46-64 in Mtb-RNase J. c The surface electrostatic potentials of regions 46–64 and 440–450 in Mtb-RNase J. d The surface electrostatic potentials of regions 46–64 and 440–470 in Mtb-RNase J. e. The interaction between regions 46–64 and 440–450. The residues were shown in stick models. f The EMSA result of Mtb-RNase J (D85A) and truncated mutants with 0.5 μM 20-nt poly (U) RNA. The asterisk indicated the bound RNA.

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