Fig. 3: dDAP-seq identifies direct target genes of C/S1 bZIPs and suggest possible functions.
From: Double DAP-seq uncovered synergistic DNA binding of interacting bZIP transcription factors
a Distribution of DAP- and dDAP-seq target gene scores for the differentially expressed genes in bzipS1 mutants. b Significance of association between DAP- and dDAP-seq target gene scores and DEGs in the bzipS1 mutant. P-values shown were calculated by the minimal hypergeometric (mHG) test for gene set enrichment without multiple comparison correction. c Comparison of enriched GO terms for predicted target genes for S1 homodimers and S1:C heterodimers. Enriched GO terms for top 2000 target genes determined from DAP- and dDAP-seq peaks of C/S1 dimers and representatives from group D (TGA5) and group S2 (bZIP3). Gray color indicates no significant association was found for that GO term (adjusted P > 0.05). Labeled boxes correspond to discussion in text. P-values plotted were computed by hypergeometric tests (one-sided Fisher exact tests) and corrected for multiple comparison by the Benjamini and Hochberg method. d Venn diagram comparing the top 2000 target genes between the S1 homodimers and S1:C heterodimers. e Significance of association between DAP- and dDAP-seq targets with DEGs in response to submergence. P-values shown were calculated by the mHG test for gene set enrichment without multiple comparison correction.
