Fig. 5: NELF-E interacts with and alters the genomic occupancy of SLUG.

a Volcano plot of NELF-E qPLEX-RIME analysis of WT+Dox vs WT-Con. Proteins that satisfy the significance threshold of |log₂(fold change)| ≥0.5 and adj. p-value <0.05 are labeled with their gene names and colored red. The p-value was adjusted by Benjamini–Hochberg multiple hypothesis correction. b Interaction network plot of proteins enriched in Dox induction by qPLEX-RIME, superimposed on the STRING interaction network. Interactions detected by qPLEX-RIME are colored in blue, while interactions from the STRING database are colored in gray. Proteins/nodes are colored based on log₂(fold change) value. c Western blot analysis of SOX9, SLUG, and NELF-E following NELF-E immunoprecipitation (IP), performed on nuclear extracts from Dox-treated MCF7ras+SS cells. Blots are representative of three independent experiments. d Genomic distribution of SLUG binding sites in MCF7ras+SS cells transduced with scrambled shRNA and treated with Dox (SCR+Dox). e Metaplots of SLUG binding across the three different categories in SCR+Dox and shNELF-E+Dox MCF7ras+SS cells. f, g Pie chart and heatmap depiction of SLUG and NELF-E co-bound peaks at TSS and distal regions. Source data are provided as a Source Data file.