Fig. 6: KAT2B is a key target of NELF-E and SLUG in response to EMT.

a Dot plot showing KAT2B as one of the top genes (n = 41) whose SLUG-mediated activation was most significantly attenuated by NELF-E KD. Expressions of corresponding genes in the two comparisons are connected by gray lines. b Genome browser tracks showing the occupancies of NELF-E, SLUG, and RNAPII on the KAT2B locus. c RT-qPCR analysis (left) and western blot analysis (right) of KAT2B in MCF7ras+SS cells treated with Dox for different time points (n = 3). d Western blot analysis of KAT2B in MCF7ras+SS cells transduced with scrambled shRNA or two independent NELF-E shRNAs and treated with or without Dox. GAPDH was used as the loading control. e Heatmap depiction of SLUG/NELF-E/KAT2B/RNAPII signals at NELF-E-alone, SLUG-alone and NELF-E/SLUG co-bound regions. f Metaplot of KAT2B ChIP-Seq signals over the different cohorts of SLUG-bound regions. g Venn diagram showing the overlap between SLUG-bound UP-genes and NELF-E/KAT2B targets. The percentage overlap of SLUG-bound UP-genes compared to other categories is also indicated. h Genome browser tracks showing the occupancies of NELF-E, SLUG, and KAT2B on LAMC1 and ZEB1 loci. Blots are representative of three independent experiments. p-values are determined by a two-tailed Student’s t-test. Mean ± SD is represented by bar graphs. Source data are provided as a Source Data file.