Fig. 3: Heterologous expression and in vivo biotransformations support NozR as a D/L DKP isomerase. | Nature Communications

Fig. 3: Heterologous expression and in vivo biotransformations support NozR as a D/L DKP isomerase.

From: Unveiling an indole alkaloid diketopiperazine biosynthetic pathway that features a unique stereoisomerase and multifunctional methyltransferase

Fig. 3

Heterologous co-expression of noz (encoding NozA CDPS and yielding 3) with nozR in S. lividans TK24 resulted in the production of cWW stereoisomers, as revealed by LC-MS using ESI+ with SIR [M + H]+ m/z 373. LC was completed using a chiral cellulose column with isocratic mobile phase of H2O/MeCN (50/50) and 0.1% HOAc, as detailed in Methods. Likewise, chemical complementation of S. lividans TK24 nozR transformants with individual cWW isomers 3, 4, or 5 resulted in observation of all three cWW stereoisomers. Source data are provided as a Source Data file.

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