Fig. 2: snRNAseq identifies three transcriptionally distinct astrocytes states in the glioma microenvironment.

a Three-dimensional tSNE plots showing all astrocyte nuclei color-coded by astrocyte state (Ast1—protoplasmic astrocytes, Ast2—reactive astrocytes with misexpression of non-astrocyte lineage genes, and Ast3—reactive astrocytes with expression of inflammatory genes. b Three-dimensional tSNE plots showing all astrocyte nuclei color-coded by disease condition. c Gene expression dot plots showing select gene marker expression for the astrocyte states. d tSNE plots showing the enrichment of gene signatures used for astrocyte clustering in astrocyte nuclei.