Fig. 3: Optimization of GGBE in diverse arrangements with HMGN1.
From: HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing
a Schematic of GGBE variants with fusion of HMGN1. b Base editing efficiency of C-to-G (left) and A-to-G (right) across GGBE1.0-GGBE1.5 at EMX1-site1 and HEK4 in HEK293T cells. c The genotypes and reads and editing frequency of indicated genotypes across GGBE1.0-GGBE1.5 at EMX1-site1. d The genotypes and reads and editing frequency of indicated genotypes across GGBE1.0-GGBE1.5 at HEK4. e Comparison of editing frequency and D-value across GGBE1.0-GGBE1.5. Total editing is the sum of editing for all positions. f Comparison of indel frequency across GGBE1.0-GGBE1.5 at EMX1-site1 (left) and HEK4 (right) in HEK293T cells. Mean ± SEM (b, e, f) of all individual values of sets of n = 3 independent replicates are shown. All statistical analysis for samples were conducted using unpaired Student’s t test (two-tailed) in GraphPad Prism 8. Source data are provided as a Source Data file.
