Table 2 Plasmids used in this study
From: Nucleotide exchange is sufficient for Hsp90 functions in vivo
Plasmid | Host | Backbone | Insert | Ref |
|---|---|---|---|---|
pMR325 | S. cerevisiae | p414-GPD | HSP82 ORF | |
pMR55W | S. cerevisiae | pRS314 | HSC82 + 500 | 60 This study |
pMR423 | S. cerevisiae | p414-GPD | hHsp90α ORF | |
pMR365 | S. cerevisiae | p414-GPD | hHsp90β ORF | |
pMR453 | S. cerevisiae | p414-GPD | CnHsp90 ORF | This study |
pMR454 | S. cerevisiae | p414-GPD | EhHsp90 ORF | This study |
pMR455 | S. cerevisiae | p414-GPD | PfHsp90 ORF | This study |
pKG2 | S. cerevisiae | p414-GPD | SpHsp90 ORF | This study |
pMR169 | S. cerevisiae | pRS313 | PGAL10::luciferase | |
pMR477 | Sz. pombe | pUR18 | hsp90+ (−669/+316) | 63 This study |
pMR496 | Sz. pombe | pSP1 | Ptdh1::MCS::TCYC1 | 64 This study |
pMR497 | Sz. pombe | pMR496 | hsp90+ ORF | This study |
pMR498 | Sz. pombe | pMR496 | HSP82 ORF | This study |
pMR499 | Sz. pombe | pMR496 | HSC82 ORF | This study |
pMR500 | Sz. pombe | pMR496 | hHsp90α ORF | This study |
pMR501 | Sz. pombe | pMR496 | hHsp90β ORF | This study |
pSK59 | E. coli | pET28b | HSP82 ORF | |
pSK92 | E. coli | pET28b | AHA1 ORF |
