Fig. 9: Adhesion, AMPK and mitochondrial dynamics in metastasis.

a (Left) Confocal images of mouse lungs 24 h after tail vein injection of 5-chloromethylfluorescein diacetate (CMFDA)-Green labelled A375M2 pre-treated with Comp C (2 μM, 24 h) and (right) percentage of field area covered by cells (15 fields/mouse/condition, 5 mice/condition, n = 2 independent experiments). Scale bar = 100 μm. b Amoeboid score comparing tumour body (TB) and invasive front (IF) in melanoma primary tumours and metastasis, calculated as indicated in methods (primary tumours n = 46, metastasis n = 42). c, d Representative QuPath mark-up images and quantification of DDR1, MFN2, OPA1, pAMPK and pMLC2 expression in matched TB and IF of metastasis from human melanoma tissue microarrays (n = 45 patients). Scale bar = 200 μm. Dot plot (a) shows median with interquartile range (each dot represents a single mouse). Box plots (b, d) show median (centre line), interquartile range (box) and min-max values (whiskers). p values were calculated using two-tailed tests (a, d). p value by Mann-Whitney test (a), one-way ANOVA with Holm-Šídák’s multiple comparisons test (b) and Wilcoxon test (d). Source data are provided as a Source Data file. See also Supplementary Fig. 9.