Fig. 6: Dimer translesion bias in XP-V skin cancers.
From: Genomic mutation landscape of skin cancers from DNA repair-deficient xeroderma pigmentosum patients
a Schematic representation of the putative CC photodimer in ACCA context and resulting mutations analyzed in the panel b. b Fraction of C > T mutations from 5’ and 3’ cytosines of the dimer in the 5’ACCA3’ context per group of tumors. Data are presented as mean values +/− SEM. n = 31 for SCC, n = 8 for BCC, n = 113 for MEL and n = 14 for XP-V (tumors). Source data are provided as a Source Data file. c “Dimer translesion bias” for different sequence contexts per group of tumors. Comparison of C > T mutation frequency in CT and TC pyrimidine dimers was performed after normalization to the number of such contexts in the genome (upper right panel). Boxes depict the interquartile range (25–75% percentile), lines - the median, whiskers − 1.5× the IQR below the first quartile and above the third quartile. n = 31 for SCC, n = 8 for BCC, n = 113 for MEL, n = 10 for XP-E, n = 8 for XP-C, n = 3 for XP-A, n = 3 for XP-D and n = 14 for XP-V (tumors). Source data are provided as a Source Data file. d Fraction of C > T mutations from 5’ and 3’ cytosines of the dimer in the 5’ACCA3’ context in the RPE-1 POLH-wt and POLH-KO clones. SEM intervals are indicated. n = 1 for NT and KbrO3 and n = 3 for UV-A and UV-C clones per cell line. Source data are provided as a Source Data file.
