Fig. 2: Vtc2 and Vtc4 interact via their SPX domains in an IPx-dependent manner. | Nature Communications

Fig. 2: Vtc2 and Vtc4 interact via their SPX domains in an IPx-dependent manner.

From: Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction

Fig. 2

a Binding affinities of Vtc2* to Vtc4*, SPX2 to Vtc4*, and SPX2 to TTM4, as determined by microscale thermophoresis (MST). b Binding affinities of SPX2 to Vtc4* in the presence of 2.5 mM IP6, 5-IP7, or 1,5-IP8 determined by MST. Cartoons illustrate the constructs used in each experiment. The resulting dissociation constants are given along with a 95% confidence interval or as a lower limit. Graphs show the means and SEM; n = 3 replicates. Source data are provided as a Source Data file.

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