Fig. 4: Improved breadth of activity of D614G/BA.1 cross-neutralizing antibodies over time.
From: Evolution of antibody immunity following Omicron BA.1 breakthrough infection
a Fab binding affinities of D614G/BA.1 cross-neutralizing antibodies isolated at 1-month12 (T1, n = 86) and 5–6-month (T2, n = 132) time points for recombinant SARS-CoV-2 variant RBDs and the SARS-CoV RBD, as determined by BLI. Black bars represent medians. b Pie charts showing the proportions of antibodies derived from (left) early12 and (right) late time points that bound the indicated number of SARS-CoV-2 variant RBDs with Fab KDs < 10 nM (statistical P = 0.026). The total number of antibodies is shown in the center of each pie. c Proportions of D614G/BA.1 cross-neutralizing antibodies from early12 (n = 86) and late (n = 132) time points with the indicated fold changes in Fab binding affinities for recombinant SARS-CoV-2 variant RBDs relative to the WT RBD. d Heatmap showing neutralization IC50s and SARS-CoV-2 variant RBD binding affinities of BA.1-specific antibodies. Statistical comparisons were determined by a, c Kruskal–Wallis test with Holms corrected multiple pairwise comparisons or b two-sided Fisher’s exact test. IC50 50% inhibitory concentration, KD equilibrium dissociation constant; *P < 0.05; **P < 0.01; ****P < 0.0001. Source data and full statistical test results are provided as a Source Data file.
