Fig. 7: Similarities and differences of tumour-infiltrating T cells between M/PDA and GSRC.

a t-SNE plot showing subclusters of T cells. b Dot plot showing the expression levels of cell type marker genes in 10 cell types. c t-SNE plot showing subclusters of T cells (coloured by cell type). d Scale plot of subclusters of T cells (n = 13). e t-SNE plots showing subclusters of T cells (coloured by group). f BOX plot of tumour-infiltrating CD4-Treg and CD8-Teff cells in M/PDA and GSRC. Compared with that in M/PDA, the proportion of tumour-infiltrating CD4-Treg cells in GSRC was higher, and the difference was not statistically significant (t-test, p = 0.25). The proportion of tumour-infiltrating CD8-Teff cells in GSRC was lower, the difference was not statistically significant (t-test, p = 0.25). n = 10 (include 3 samples in MDA and PDA groups, respectively; and 4 samples in GSRC group). Data are presented as mean values ± SEM. In the box plot, the black dots represent outliers, the error bars represent SEM, the box midpoints represent means and the boxes represent inter-quartile positions. P values were calculated using the two-side unpaired Student’s t-test. g Differential plot of tumour-infiltrating CD4-Treg and CD8-Teff cells in M/PDA and GSRC by IHC score (n = 30). Compared with that of M/PDA, the IHC score of tumour-infiltrating CD4-Treg cells (expressing FOXP3) in GSRC was higher, and the difference was statistically significant (Wilcoxon rank-sum test, p = 0.0032); The IHC score of tumour-infiltrating CD8-Teff cells (expressing KLRD1) was lower in GSRC, and the difference was statistically significant (Wilcoxon rank-sum test, p = 0.0021). p values were calculated using the Wilcoxon rank-sum test. p values were calculated using the Wilcoxon rank-sum test. p values < 0.05 were considered to indicate significance: **p < 0.01; ns: no significance. Source data are provided as a Source Data file.