Fig. 3: Microtubule-mediated lysosome trafficking shuttles RNA. | Nature Communications

Fig. 3: Microtubule-mediated lysosome trafficking shuttles RNA.

From: Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo

Fig. 3

a 3D view of dividing 8- to 16-cell stage embryo labelled with RNA, LAMP1-3xeGFP and BFP-Utrophin (top). Merged (middle) and single channels (bottom) show segmented and masked outer blastomere (insets) with apical-to-basal border indicated (dashed line). White arrowheads indicate basal co-enrichment. b Quantification of LAMP1-3xeGFP fluorescence expression at 8- and 16-cell stages. c Proximity of RNA (white arrowheads) and LAMP1-3xeGFP positive vesicular structures (orange arrowheads) at 8-cell stage and d late 16-cell stage. e High spatiotemporal resolution time-series imaging of RNA (grey arrowheads) and LysoSensor-positive vesicular structures (orange arrowheads) during co-trafficking events. f At 16-cell stage Annexin A11 (ANXA11-mEmerald) tethers RNA and LAMP1-positive vesicular structures (blue arrowhead) and g is present between LAMP1-positive vesicular-like structures and microtubule filaments (blue arrowheads). h Apicobasal RNA distribution in control and Annexin A11 siRNA-microinjected embryos. i Developmental rate of control, Annexin A11 siRNA-, Borcs7 siRNA- and Annexin-R346C mutant-microinjected embryos. Data are represented as a percentages (mean); n = embryos. j Apicobasal RNA distribution in control, Borcs7 siRNA- and Annexin-R346C mutant-microinjected embryos. Box plots in b, h and j display minimum, lower quartile, median, upper quartile and maximum. Unpaired Mann-Whitney or Welch’s two-tailed t-tests were used to identify statistical differences. Scale bars 10 μm; 1 μm insets; except insets in (a) 5 μm. Source data are provided as a Source data file.

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