Fig. 4: RNA subtypes required for protein synthesis are enriched apically in late 16-cell stage outer blastomeres.
From: Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo
Late 16-cell stage embryos show an apical enrichment (white arrowheads) for a, b mRNA (Poly(A) RNA FISH) and c, d tRNA FISH. e, f Global RNA and ribosomal RNA (rRNA-naphthalimide) co-clusters in apical (white arrowheads) and basal (orange arrowheads) regions of late 16-cell stage blastomeres. g Small 40S (RPS6-mScarlet) and large 60S ribosomal subunit (L10A-eGFP) expression in apical (white arrowheads) and basal (orange arrowheads) regions. h Increased basal RNA-binding protein HTATSF1 (HTATSF1-mScarlet) (orange arrowheads) distribution with L10A-eGFP co-labelling (white arrowheads) in 16-cell stage outer blastomere. Data in b, d and f are presented as mean ± SEM; thick lines indicate mean, transparent shadows depict SEM. Scale bars 10 μm. Source data are provided as a Source data file.
