Fig. 7: Biased inheritance of RNA following asymmetric cell divisions from 16- to 32-cell stage.
From: Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo
a Symmetric cell division of dividing 16- to 32-cell stage embryo labelled for RNA and Membrane-GFP. b RNA intensity profiles from 16-cell stage mother cells (pre-division) to outer 32-cell stage daughter cells during symmetric cell divisions (dashed vertical line); bold line indicates mean of individual tracked divisions (faint grey lines). c Segmentation of tracked blastomeres (grey) in (a), ~6.5 h following symmetric cell divisions and d correlative immunostaining of CDX2 with close-up view of outer daughter cells (grey arrowheads). e Asymmetric cell division of dividing 16- to 32-cell stage embryo labelled for RNA and Membrane-GFP. f RNA intensity profiles of inner sister (orange lines) and outer sister blastomeres (faint grey lines) following asymmetric cell divisions; bold line indicates mean values. g Segmentation of tracked blastomeres in e, 8 h 20 min following asymmetric cell division demonstrates outer (grey) and inner (orange) positions and h correlative immunostaining of CDX2 with close-up view of outer (grey arrowhead) and inner (orange arrowhead) daughter cell. Scale bars 10 μm; insets 5 μm. Source data are provided as a Source data file.
