Fig. 8: ER is preferentially inherited by and enriched in outer cells at 32-cell stage.
From: Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo
a Partial redistribution of ribosomes (L10A-eGFP) during 16- to 32-cell stage asymmetric divisions. b Quantification of ribosome inheritance amongst inner and outer sister blastomeres. c Biased inheritance of endoplasmic reticulum (Emerald-Sec61β) following 16- to 32-cell stage asymmetric divisions. Prospective outer blastomere (grey arrowhead) displays higher ER intensity (insets). d Quantification of (c). e Serial blockface scanning electron microscopy (SBF-SEM) images of 32-cell stage embryo show ER filament-like structures (red dashed inset) surrounding cytoplasmic lattice structures in outer blastomeres (green dashed inset). f Quantification of ER filaments from SBF-SEM images. Box plots in b, d and f display minimum, lower quartile, median, upper quartile and maximum. Unpaired Mann-Whitney two-tailed t-tests were used to identify statistical differences. Individual data points are overlaid in (f). Scale bars 10 μm; insets 5 μm. SBF-SEM scale bars 0.5 μm. Source data are provided as a Source data file.
