Fig. 9: Pluripotent inner cells require lower translation capacity compared to differentiating outer trophectoderm cells.
From: Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo
a Immunofluorescence shows blastomeres positive for trophectoderm marker CDX2 (grey arrowheads) with higher SEC61β intensity compared to SOX2 positive blastomeres (orange arrowheads) and CDX2/SOX2 negative blastomeres (purple arrowheads). b Quantification of SEC61β fluorescence intensity. c SOX2 and CDX2 expression in control and Annexin siRNA-treated embryos. d Quantification of nuclear (nuc) and cytoplasmic (cyto) SOX2 fluorescence intensity. e Inner (orange) and outer (grey) blastomere numbers in control and Annexin A11 siRNA-treated embryos after 5th cell division. n = embryos. Box plots in b, d and e display minimum, lower quartile, median, upper quartile and maximum. Unpaired Mann–Whitney or Welch’s two-tailed t-tests were used to identify statistical differences. Scale bars 10 μm; insets 5 μm. Source data are provided as a Source data file.
