Fig. 6: Stat1 is required for MCMV genome persistence in fibroblastic cells in vivo.
a–d Analysis of the lytic infection of red pulp FC in WT and Stat1−/− mice 22 h after the administration of MCMVΔgL-GFP intraperitoneally or intravenously. a Experimental design for (b–d). b–d Percentage of GFP+ cells in red pulp FC. b Representative flow cytometry plot. Numbers indicate the percentage of gated cells. c, d Data are presented as the arithmetic mean ± SD of n = 3 biologically independent mice (depicted as symbols) examined over one experiment performed for each infection route. *p-value < 0.05 (exact p value indicated in the plot), two-tailed unpaired t test. e–g Analysis of latent MCMV persistence in red pulp FC from WT and Stat1−/− mice 60 days after intraperitoneal administration of MCMVΔgL-GFP. e Experimental design for (f, g). f Plots show the lack of GFP+ cells in red pulp FC. g Number of virus genomes per 100,000 of red pulp FC. Horizontal line depicts arithmetic mean ± SD from n = 4 biologically independent samples (depicted as symbols) examined over two independent experiments. Biological replicates represent cells sorted from pooled preparations from two mice. **p-value < 0.01 (exact p value indicated in the plot), two-tailed Welch’s t test. q.l. quantification limit, n.d. not detected. Source data are provided as a Source Data file.
