Table 1 Quantification of viral mRNAs in cell populations from latently infected mice by RT-qPCR
Type of infection | Cell type | Estimated number of cells per reaction | MCMV transcript copies detected per reaction | |||||
|---|---|---|---|---|---|---|---|---|
ie1/3 | M38 | M48(SCP) | ||||||
5 months after intraperitoneal infection | Spleen PDGFRα+ FC | 8911 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
9153 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
7630 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
Visceral adipose tissue PDGFRα+ FC | 8359 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
7659 | 21 | 8 | n.d. | n.d. | n.d. | n.d. | ||
11,799 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
Salivary gland PDGFRα+ FC | 5468 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
6242 | 123 | 140 | n.d. | n.d. | n.d. | n.d. | ||
6242 | n.d. | n.d. | n.d. | 9 | 58 | 60 | ||
Liver PDGFRα+ FC | 6224 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
6735 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
6715 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
Liver EC | 9333 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
6618 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
9488 | 8 | 7 | n.d. | n.d. | n.d. | n.d. | ||
Lung PDGFRα+ FC (negative control) | 14,431 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
15,234 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
16,971 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
5 months after intranasal infection | Lung PDGFRα+ FC | 16,971 | 200 | 128 | n.d. | n.d. | n.d. | n.d. |
14,756 | 405 | 303 | 7 | 16 | n.d. | n.d. | ||
17,261 | 317 | 433 | n.d. | n.d. | n.d. | n.d. | ||
3 months after intraperitoneal infection | Visceral adipose tissue PDGFRα+ FC | 10,626 | 10 | 14 | n.d. | n.d. | n.d. | n.d. |
11,751 | 11 | 29 | n.d. | n.d. | n.d. | n.d. | ||
9,498 | 40 | 64 | n.d. | n.d. | n.d. | n.d. | ||
Visceral adipose tissue Mϕ | 10,551 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
12,228 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
10,975 | 5 | 5 | n.d. | n.d. | n.d. | n.d. | ||
