Table 2 Quantification of viral mRNAs in cell populations from acutely infected mice by RT-qPCR

From: Fibroblasts are a site of murine cytomegalovirus lytic replication and Stat1-dependent latent persistence in vivo

Type of infection

Cell type

Estimated number of cells per reaction

MCMV transcript copies detected per reaction

ie1/3

M38

M48(SCP)

12 h after intraperitoneal infection

Spleen PDGFRα+ FC

7944

219,224

211,938

411,485

411,485

61,694

61,289

Uninfected

Spleen PDGFRα+ FC

6113

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

  1. cDNA isolated from pooled cell preparations from 4 mice was analysed in 2 technical duplicates per MCMV gene.
  2. n.d. not detected, MCMV murine cytomegalovirus, FC fibroblastic cells.