Fig. 4: Recombinant S. alvi M0351 strains engineered to deliver dsRNA inhibit the infection of N. ceranae. | Nature Communications

Fig. 4: Recombinant S. alvi M0351 strains engineered to deliver dsRNA inhibit the infection of N. ceranae.

From: Engineered symbiotic bacteria interfering Nosema redox system inhibit microsporidia parasitism in honeybees

Fig. 4

a Newly emerged bees were colonized with engineered S. alvi and orally infected with N. ceranae. b–g Engineered S. alvi strains to produce dsRNA targeting genes of the thioredoxin and glutathione systems inhibited the gene expression of N. ceranae in the midgut (n = 5). Two-sided Mann–Whitney U test. h, i Inhibition of N. ceranae infection by engineered S. alvi M0351 strains. Nosema infection was assessed by both spore counts (h) (n = 8 bees for all groups) and qPCR (i) (n = 10 bees for all groups). The overall significance was first assessed by one-sided ANOVA (p < 0.01). Brown–Forsythe and Welch test was used for multiple comparisons, the letters above each bar stand for statistical differences among the treatments (p < 0.05). Error bars represent mean SD. Source data are provided as a Source Data file.

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