Fig. 1: Using IL23-TMR to characterise IL-23 receptor antagonists.
From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes
a A schematic demonstrated the principles of the BRET competition assay using IL12p40 as an example. Schematic created with Biorender.com. IL23R is shown in orange, IL12Rβ1 in purple, IL23p19 in blue, IL12p40 in green and the presence of a TAMRA fluorophore by a pink star. The competitive inhibition of the binding of 300 pM IL23-TMR by increasing concentrations of (b) endogenously produced IL-12p40 containing proteins and (c) synthetic IL-23 receptor antagonists with previously generated IL-23 competition data24 for comparison. d The combined data from b and c plotted without error bars. In b and c, data are mean ± SEM from 5 independent experiments conducted in either duplicate or triplicate. Details on absolute number of data points included for each concentration are provided in the associated Source Data file. Ki values obtained in the 5 independent experiments are shown in Table 2.
