Fig. 2: Creation and characterisation of a fluorescent cyclic peptide probe for IL23R.
From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes
The chemical structures of (a) P630 and (b) P630-TMR. The BRET ratio measured when increasing concentrations of P630-TMR were applied to cells expressing (c) NL-IL23R alone, (d) NL-IL23R with IL12Rβ1 or (e) NL- IL12Rβ1 and IL23R in the presence or absence of 10 μM P630. f The data shown in c–e normalised to % maximum BRET ratio. g The BRET data shown in c–e with non-specific binding subtracted. h The specific binding of P630-TMR to cells expressing NL- IL12Rβ1 in the presence or absence of IL23R. Data are normally mean ± SEM from five independent experiments. Details on absolute number of data points included for each concentration are provided in the associated Source Data file. In each individual saturation experiment triplicate determinations were normally made for total binding and duplicate measurements were made for non-specific binding. Kd values obtained in the 5 independent experiments are shown in Table 2.
