Fig. 3: P630-TMR binding to binary NanoBiT IL-23 receptor complexes.
From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes
a A schematic depicting the NanoBiT assay methodology, with IL23R shown in orange and IL12Rβ1 shown in purple. Schematic created with Biorender.com. b The BRET ratio generated when increasing concentrations of P630-TMR were applied to cells expressing NanoBiT complemented receptors. c The effect of P630 on NanoLuc complementation and hence receptor dimerisation. d The data from (b) normalised to % of P630-TMR specific Bmax and plotted with data generated in Fig. 2d, e for reference. Data in b are mean ± SEM from three independent experiments. In each individual experiment, triplicate determinations were made for total binding and duplicate or triplicate determinations made for non-specific binding. Data in c are the mean values obtained in five independent experiments, each conducted with six replicates. Ns no significant difference (two-tailed, paired t test of the paired mean values obtained in each experiment; p = >0.05). Data in d are the mean ± SEM from five (NL-IL23R and NL-23R + IL12Rβ1) or three (HiBit-IL23R + LgBit-IL12Rβ1) independent experiments. Source data are provided as a Source Data file.
