Fig. 4: The displacement of P630-TMR by IL-23 is dependent on the co-expression of IL12Rβ1 with IL23R.
From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes
a The displacement of 75 nM P630-TMR by increasing concentrations of IL-23 from cells expressing NL-IL23R in the presence or absence of IL12Rβ1. b The displacement of different concentrations of IL23-TMR by increasing concentrations of P630. c The data shown in b normalised to % inhibition (total inhibition was defined by an excess of IL-23). d The relationship between P630 IC50 determined in c and the concentration of IL23-TMR used. Data are mean ± SEM from four (a: NL-IL23R + pc3.1 zeo) or five independent experiments performed in triplicate. In b the bars show the mean values obtained in each of five independent experiments and the data points show the mean ± SEM of the values obtained in the five independent experiments. Source data are provided as a Source Data file.
