Fig. 5: P630-TMR measures antagonism at the IL23R/IL23p19 interface.
From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes
a The displacement of 75 nM P630-TMR by increasing concentrations of various IL-23 receptor binders. b The displacement of either 75 nM P630-TMR or 300 pM IL23-TMR by a single concentration of several antagonists. Data in a are mean ± SEM from three (TEEEQQLY), four (P630, IL12p40) or five (IL-23, IL12p80) independent experiments performed in triplicate. Data in b are mean ± SEM from five independent experiments performed in triplicate apart from 200 nM IL23p19 (4 experiments) and 500 nM IL12p40 with P630-TMR (4 experiments). In b statistical analysis (two-tailed, non-paired, t-test) was performed on the mean values obtained in each independent experiment. ** indicates a statistical significance of p < 0.01. *** indicates a statistical significance of p < 0.001. Absolute p values in b were 0.000001 (IL-12p40), 0.000002 (IL12p80), 0.0069 (IL12) and 0.74 (IL23p19). Source data are provided as a Source Data file.
