Table 1 LS-MS/MS profiles for labelled (TMR) and unlabelled P630

From: Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes

Peptide

Mass/charge (m/z)

Charge (z)

Mono-isotopic mass (M + H)a

Retention time (min)

Purity (%)

P630-TMR

1103.96

2

2206.92

10.53

>95

P630

833.30

2

1665.60

9.92

>95

  1. aMono-isotopic mass at a single charge assuming the peptide takes up a single proton. Mono-isotopic mass [M + H] is calculated as (m/z)*z-(z-1). The difference in mono-isotopic mass between P630-TMR and P630 is 541.32 which is equivalent to the theoretic mass of 541.45 for the addition of 5-carboxytetramethylrhodamine and lysine to the C-terminus of P630.
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