Fig. 7: Source of R919* TRPA1-mediated channel hyperactivity.
From: Molecular mechanism of hyperactivation conferred by a truncation of TRPA1
a Linear diagram depicting major structural domains in a WT hTRPA1 monomer. Missing portions from all C-terminal truncation constructs denoted above. R919* truncation completeness scales with gradations of pink. b Ratiometric calcium imaging of HEK293T cells transiently co-transfected with 3×FLAG-WT hTRPA1 and empty vector (mock) or the indicated C-terminal truncation constructs. Cells were stimulated with 10 µM AITC. Scale bars indicate 100 µm. Data representative of four independent experiments. c Quantification of 10 µM AITC-evoked change in Fura-2 ratio relative to maximum response of each expression condition at 100 µM AITC. Data further normalized to WT hTRPA1 response and WT hTRPA1 expression. Colors as indicated in a. Data represent mean ± SEM. ****p < 0.0001, **p < 0.01, n.s. not significant. n = 4 independent experiments, n ≥ 60 cells per transfection condition per experiment, one-way ANOVA with Tukey’s post hoc analysis. Source data are provided as a Source Data file.
