Fig. 5: HTNV-L in elongation state.
a Global view of HTNV-L elongation state. The density corresponding to the mutated 5′vRNA (5′mut), the 3′vRNA1–25 and the product RNA are displayed in pink, yellow, and cyan respectively. Three different chains of 3′vRNA1–25 are visible due to the excess of 3′vRNA1–25 in the elongation reaction mix. Their nature is indicated. b Schematic organization of the 5′mut, 3′vRNA1–25 and product RNA. The representation is the same as in Fig. 4b. c Zoom on the active site at elongation. The motifs and the putative prime-and-realign (PR) loop are shown and colored as in Fig. 2f. The template and the product are shown as yellow and cyan cartoons. The position of the active site that corresponds to the next nucleotide to be incorporated is labeled as +1. Important residues of the motifs and the putative PR loop are shown as stick and labeled. The magnesium ion is shown as a green dot. d Zoom on the template/product duplex in the active site cavity. The template and the product are shown as yellow and cyan cartoons with nucleotides labeled. HTNV-L regions that interact are displayed as cartoon and colored as in Fig. 1. Residues in interaction are shown as sticks and labeled. The template and product exits are shown with arrows. e Schematic representation of the HTNV-L/template/product interactions. HTNV-L residues that bind to the template and the products labeled are colored according to the domain to which they belong. Hydrogen bond and polar interactions are respectively shown as black and red lines. Position +1 of the active site is indicated. The lid domain and in particular its residue Y1564 that separates the template/product duplex is shown.
