Fig. 6: NDP52 changes DNA conformation in vitro.
From: Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription
A AFM image of ds339 on PLO in the absence of NDP52. Scale bar = 100 nm. Height scale = 4.5 nm. B AFM image of ds339 on PLO with NDP52. Scale bar = 100 nm. Height scale = 4.5 nm. C AFM images of NDP52-FL bridging strands of linear (339 bp) dsDNA on poly-L-ornithine surface. Scale bar = 100 nm. Height scale = 4.5 nm. D Normalised histogram comparing the maximum bounding size of DNA with (light blue) and without NDP52 (orange); their distributions overlap (dark blue). E Cropped AFM images of DNA molecules in the presence of NDP52 on PLO. First two images are from between 50–60 nm maximum bounding size while the second two are between 80–90 nm. F AFM images showing bridging between DNA molecules in the presence of NDP52 on PLO. xy scale bars 100 nm. G Preference for NDP52-FL binding on ds339 molecule. Diagram shows ds339 edge and middle references on linear DNA. Violin plot shows %distance from DNA edge values for NDP52-FL binding. Mean ± SEM is shown for n = 270 binding events. Raw data are available in the Source Data file.
