Fig. 2: Reducing SV release probability does not affect coding reliability at low stimulus intensities.
From: Homeostatic synaptic plasticity rescues neural coding reliability
A PSTH of PN population responses to three low concentration odors examined as indicated (shaded areas represent SEM, odor pulse is labeled with a black bar). Spike trains were binned using a 50 ms time bin. Knockdown of cac (green) in ORNs did not affect PN odor responses. A final odor dilution of 5 × 10−4 was used (n = 50 flies). Orco-GAL4 drove the RNAi construct and GH146-QF drove QUAS-GFP. B, C Temporal reliability analysis for data in panel A. cacRNAi in ORNs did not reduce correlation values. Two-sided permutation test, p < 0.001. D Firing-rate reliability analysis for data in panel A. cacRNAi in ORNs did not increase firing rate variability. Error bands represent SEM. E, F Temporal reliability analysis for data in Fig. S1. Removing the first 200 ms of the odor response leads to an overall decrease in correlation. A shift of the peak of the curve is observed for the sustained odor response in cacRNAi flies. Two-sided permutation test, p < 0.001. G Firing-rate reliability analysis for data in Fig. S1 without the first 200 ms of the odor response. Contrary to the increased variation observed for the entire odor response (Fig. 1F), cacRNAi in ORNs did not increase the variability for the sustained odor response although high firing-rates were still obtained. Error bands represent SEM. H The ratio between the area under the curve (AUC) of the rate code variability in cacRNAi relative to wt. The initial response was progressively cropped from the analysis. The difference in rate code variation of wt and cacRNAi flies gradually decreases over the first 200 ms of the odor response. For all panels ***p < 0.001, for detailed statistical analysis, see Table S1.
