Fig. 3: Presynaptic cac knockdown induces homeostatic plasticity.
From: Homeostatic synaptic plasticity rescues neural coding reliability
A Experimental scheme. UAS-cacRNAi was expressed in ORNs using Orco-GAL4 and whole-cell patch clamp recordings were made from PNs labeled by the GH146-QF driver line. The antennal nerve was stimulated with a suction electrode. B The average eEPSC amplitude was unaltered by cacRNAi for 2–4-day-old flies (1 Hz stimulation frequency; wt n = 17 and cacRNAi n = 18 flies). Two-sided permutation test, p = 0.789. C Examples traces of eEPSC evoked by paired-pulse stimulation with an inter-pulse interval of 10 ms. D Quantification of the paired-pulse ratio at different inter-stimulus intervals for 2–4-day-old flies (10 ms, 30 ms, 100 ms, 300 ms, and 1000 ms). cacRNAi significantly increased paired-pulse facilitation at short inter-pulse intervals [wt, n = 20 (10 ms), n = 21 (30 ms), n = 22 (100, 300, 1000 ms); cacRNAi, n = 25 (10 ms), n = 24 (30 ms), n = 28 flies (100, 300, 1000 ms)]. Error bars represent SEM. Two-sided permutation test, p = 0.01 (10 ms), p = 0.034 (30 ms). E, F Average eEPSC latencies (E) and average eEPSC jitter (F) at 1, 10, 20, and 60 Hz stimulation for wt (n = 17) and cacRNAi (n = 18) flies. A significant increase was observed for all tested stimulation frequencies. Two-sided permutation test and two-sided two sample t-test, p = 0.001 (1 Hz latency), p < 0.001 (10 Hz latency), p < 0.001 (20 Hz latency), p < 0.001 (60 Hz latency and jitter), p = 0.044 (1 Hz jitter), p = 0.016 (10 Hz jitter), p = 0.01 (20 Hz jitter). G The average eEPSC amplitude was reduced by cacRNAi for 0-day-old flies (1 Hz stimulation frequency; wt n = 20 and cacRNAi n = 19 flies). Two-sided permutation test, p = 0.016. H Quantification of the paired-pulse ratio at different inter-stimulus intervals for 0-day-old flies (10 ms, 30 ms, 100 ms, 300 ms, and 1000 ms). cacRNAi significantly increased paired-pulse facilitation at short inter-pulse interval [wt, n = 20 (10 ms), n = 21 (30, 100, 300, 1000 ms); cacRNAi, n = 19 flies]. Error bars represent SEM. Two-sided permutation test, p = 0.025. I The average eEPSC amplitude was unaltered by cacRNAi for 1-day-old flies (1 Hz stimulation frequency; wt n = 17 and cacRNAi n = 18 flies). J Quantification of the paired-pulse ratio at different inter-stimulus intervals for 1-day-old flies (10 ms, 30 ms, 100 ms, 300 ms, and 1000 ms). cacRNAi significantly increased paired-pulse facilitation at short inter-pulse interval [wt, n = 20 (10 ms), n = 21 (30 ms), n = 22 (100, 300, 1000 ms); cacRNAi, n = 22 flies]. Error bars represent SEM. Two-sided permutation test, p < 0.001. For all panels *p < 0.05, **p < 0.01, ***p < 0.001, for detailed statistical analysis, see Table S1.
