Fig. 4: Neural coding reliability is not rescued by homeostatic plasticity in newly eclosed flies.
From: Homeostatic synaptic plasticity rescues neural coding reliability
A Experimental scheme. UAS-cacRNAi was expressed in ORNs using Orco-GAL4 and whole-cell patch clamp recordings were made from PNs labeled by the GH146-QF driver line. PN responses were measured for both high and low odor concentrations in 0-day-old flies. B PSTH of PN population responses to five high concentration odors examined as indicated (shaded areas represent SEM, odor pulse is labeled with a black bar) in 0-day-old flies. Spike trains were binned using a 50 ms time bin. Knockdown of cac (green) in ORNs resulted in decreased PN odor responses. A final odor dilution of 5 × 10−2 was used (n = 50–57 flies). Orco-GAL4 drove the RNAi construct and GH146-QF drove QUAS-GFP. C, D. Temporal reliability analysis (as in Fig. 1D, E) for data in panel B. cacRNAi in ORNs reduced correlation values. Two-sided permutation test, p < 0.001. E Firing-rate reliability analysis (as in Fig. 1F) for data in B. cacRNAi in ORNs increased firing rate variability. Error bands represent SEM. F PSTH of PN population responses to three low concentration odors examined as indicated (shaded areas represent SEM, odor pulse is labeled with a black bar) in 0-day-old flies. Spike trains were binned using a 50 ms time bin. Knockdown of cac (green) in ORNs resulted in decreased PN odor responses. A final odor dilution of 5 × 10−4 was used (n = 49–50 flies). Orco-GAL4 drove the RNAi construct and GH146-QF drove QUAS-GFP. G Temporal reliability analysis (as in Fig. 1D, E) for data in panel F. cacRNAi in ORNs reduced correlation values. Two-sided permutation test, p < 0.001. For all panels ***p < 0.001, for detailed statistical analysis, see Table S1.
