Fig. 2: Diverse transcripts in the cochlea.

a The identified isoforms were categorized as follows: FSM (full splice match), ISM (incomplete splice match), NIC (novel in catalog), and NINC (novel not in catalog). b Pie charts showing the number of different categories of isoforms and genes from ScISOr-Seq. c The number of novel isoforms detected per gene by ScISOr-Seq showed higher isoform diversity than that in the mouse cerebellum and that determined by Ensembl annotation. d Distribution of the identified transcript isoform categories across cochlea cell types. e UpSet plot comparing the shared novel transcripts among different cell types. Most of the novel transcripts exhibited cell-type specificity. f Actb expression level among the different cell types, measured by short-read sequencing (cells with high expression are in red). g The number of novel transcripts detected by long-read sequencing (the number in the brackets refers to the cell-type specificity transcript number). h Actb transcripts were identified by ScISOr-Seq and revealed high diversity and cell-type specificity. Each colored row represents the identified transcripts according to each cell type. Blocks represent exons, while the white space represents intronic space. The black track shows the GENCODE annotation of Actb (bottom). Arrow: a newly identified exon.